1. What is the SINGUWAY STI 18 Nucleic Acid Detection Kit (STD018)?

The SINGUWAY STI 18 Kit (STD018) is a multiplex real-time PCR assay designed for the qualitative detection of 18 sexually transmitted infection-related pathogens. It supports molecular testing of urethral swab and genital tract secretion swab specimens in professional diagnostic laboratories.

2. Which pathogens can the STI 18 kit detect?
The kit detects 18 targets, including:
Ureaplasma parvum (UP)
Neisseria gonorrhoeae (NG)
Atopobium vaginae (AV)
Treponema pallidum (TP)
Herpes simplex virus type 1 (HSV-1)
Herpes simplex virus type 2 (HSV-2)
Mycoplasma genitalium (MG)
Haemophilus ducreyi (HD)
Chlamydia trachomatis (CT)
Candida albicans (CA)
Gardnerella vaginalis (GV)
Varicella-Zoster virus (VZV)
Ureaplasma urealyticum (UU)
Mycoplasma hominis (MH)
Trichomonas vaginalis (TV)
Group B Streptococcus (GBS)
Mobiluncus species (Mob) 
Schistosoma haematobium (SH)

3. What are the clinical applications of the STI 18 kit?
The kit is designed to support molecular testing in hospital laboratories, specialized STI laboratories and professional diagnostic centers.
Its broad pathogen coverage may support the laboratory investigation of patients with suspected sexually transmitted infections, including cases involving urethral or genital tract symptoms.
Test results should be interpreted together with clinical findings and other laboratory information.

4. What are the main advantages of the STI 18 multiplex PCR kit?
Key features include:
- 18-target coverage: Detects a broad range of STI-related bacterial, viral, fungal and protozoan pathogens.
- Multiplex real-time PCR:Supports multiple pathogen detections within one testing workflow.
- Four fluorescence channels:Uses FAM, HEX, ROX and CY5.
- Internal control:Included to support monitoring of the testing process.
- Contamination control: Incorporates a dUTP-UNG approach.
- Flexible packaging:Available in 24-test and 48-test configurations.

5. What specimen types are suitable for the STD018 kit?
The kit is designed for urethral swab and genital tract secretion swab specimens. Specimen collection, handling and processing should follow the instructions for use.

6. How does the five-tube reaction design work?
The 18 pathogen targets are distributed across five PCR reaction tubes. Each specimen therefore requires five PCR reactions.
The assay uses four fluorescence channels: FAM, HEX, ROX and CY5. An internal control is also included according to the kit's reaction design.

7. Which real-time PCR instruments are compatible with the kit?
The STD018 kit is compatible with selected real-time PCR instruments, including:
- SINGUWAY AccuRa-32
- SINGUWAY AccuRa Mini
- Bio-Rad CFX96
- Applied Biosystems ABI 7500
- Roche LightCycler 480
Compatible instruments must support the required four fluorescence channels and assay configuration.

8. What is the analytical sensitivity of the STI 18 kit?
The analytical sensitivity specified for the STD018 kit is 1,000 copies/mL. 

9. How is the STI 18 kit used in a molecular testing workflow?
A typical workflow includes specimen collection, nucleic acid extraction, preparation of five PCR reactions, real-time PCR amplification and result analysis.
For each reaction, 10 μL of extracted nucleic acid is added as the template according to the instructions for use.

10. Can the STI 18 kit be integrated with SINGUWAY instruments?

Yes. The STD018 kit can be used with compatible SINGUWAY real-time PCR systems, including AccuRa-32 and AccuRa Mini.
Combined with a suitable nucleic acid extraction workflow, it provides laboratories with an option for integrating sample preparation and downstream molecular detection.
The kit is intended for professional in vitro diagnostic use. Results are for clinical reference and should not be used as the sole basis for diagnosis or treatment decisions.