

Intended Use
Mosquito-borne diseases, generally referred to as diseases that are transmitted by mosquitoes as transmission vectors, spread widely in tropical/subtropical countries; Among them, dengue virus, Zika virus and chikungunya virus have a wide transmission ability, causing various hemorrhagic fevers; Therefore, diagnosis is mandatory.
TheDetection Kit for dengue virus/Zika virus/ chikungunya virus (PCR-Fluorescence Probing) is an in vitro diagnostic test, based on real-time PCR technology, for the detection ofdengue virus/Zika virus/ chikungunya virus nucleic acids inserum samples. The test results are for clinical reference only and cannot be used as a basis for confirming or excluding cases alone.
	
Test Principle
This kit is based on real-time PCR fluorescence technology, and the specific primers and corresponding fluorescent probes target to the conserved region ofdengue virus/Zika virus/ chikungunya virus were designed for highly specific detection. By detecting the fluorescent signals changes, thedengue virus/Zika virus/ chikungunya virus nucleic acid from specimen can be qualitatively detected.
	
Technical Specifications
| REF No. | MBD001 | |||
| Regulatory Status | CE IVD | |||
| Packing Specifications | 48 tests/kit, 96 tests/kit | |||
| Technology | Real-time PCR | |||
| Type Of Analysis | Qualitative | |||
| Sample Type | Serum | |||
| Reaction Volume | 30μL | |||
| Detecting Target | 
					 DENV/CIKV/ZIKV 
					 | |||
| Analytical Sensitivity | 1000 copies/mL | |||
| Precision | CV≤5% | |||
| Storage | -20±5℃ in dark for 12 months 2~8℃ after opening the cover for2 weeks | |||
| Validated Extraction Method | General nucleic acid extraction kit (Singuway Ref No.: MTQ064/MTQ096) is recommended. | |||
| Instruments | 
					 A real-time PCR instrument containing FAM, HEX/VIC, ROX and Cy5 fluorescence channels, such as Singuway Singu9600, Bio-Rad CFX96, ABI 7500. 
					 | |||
| Required Detection Channels | FAM, HEX, CY5, ROX | |||